This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Métris, A.
Right arrow Articles by Baranyi, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Métris, A.
Right arrow Articles by Baranyi, J.
Agricola
Right arrow Articles by Métris, A.
Right arrow Articles by Baranyi, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2006, p. 6674-6679, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00914-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of Optical Density Detection Times To Assess the Effect of Acetic Acid on Single-Cell Kinetics{triangledown}

A. Métris,* S. M. George, and J. Baranyi

Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, United Kingdom

Received 18 April 2006/ Accepted 10 August 2006

The growth of Listeria innocua at different acetic acid concentrations (0 to 2,000 ppm) was monitored by optical density measurements in a Bioscreen (Labsystems, Vantaa, Finland). The generated populations came from low inocula that were obtained by serial dilution. A new method to estimate both the growth rate and the lag time of single cells from the detection times (time to reach an optical density of 0.11) was developed. It assumes that the single-cell lag times follow a gamma distribution and takes into account the randomness of the inoculation level. (The initial cell number per well was assumed to follow a Poisson distribution.) In this way, relatively small numbers of replicates are sufficient to obtain a robust estimation of the distribution of single-cell lag times. The results were validated with plate count experiments. It was found that logarithms of both the growth rates and of population lag times increased linearly with the acetic acid concentration. The logarithm of the scale parameter of the gamma distribution of the single-cell lag times also increased linearly with the acetic acid concentration irrespective of the phase of the inoculum.

* Corresponding author. Mailing address: Institute of Food Research, NRP, Norwich NR4 7UA, United Kingdom. Phone: 44 1603 255 021. Fax: 44 1603 255 288. E-mail: aline.metris{at}bbsrc.ac.uk.

{triangledown} Published ahead of print on 1 September 2006.

Applied and Environmental Microbiology, October 2006, p. 6674-6679, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00914-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aguirre, J. S., Pin, C., Rodriguez, M. R., Garcia de Fernando, G. D. (2009). Analysis of the Variability in the Number of Viable Bacteria after Mild Heat Treatment of Food. Appl. Environ. Microbiol. 75: 6992-6997 [Abstract] [Full Text]  
  • Dupont, C., Augustin, J.-C. (2009). Influence of Stress on Single-Cell Lag Time and Growth Probability for Listeria monocytogenes in Half Fraser Broth. Appl. Environ. Microbiol. 75: 3069-3076 [Abstract] [Full Text]  
  • Metris, A., George, S. M., Mackey, B. M., Baranyi, J. (2008). Modeling the Variability of Single-Cell Lag Times for Listeria innocua Populations after Sublethal and Lethal Heat Treatments. Appl. Environ. Microbiol. 74: 6949-6955 [Abstract] [Full Text]  
  • Niven, G. W., Morton, J. S., Fuks, T., Mackey, B. M. (2008). Influence of Environmental Stress on Distributions of Times to First Division in Escherichia coli Populations, as Determined by Digital-Image Analysis of Individual Cells. Appl. Environ. Microbiol. 74: 3757-3763 [Abstract] [Full Text]  
  • Pin, C., Baranyi, J. (2008). Single-Cell and Population Lag Times as a Function of Cell Age. Appl. Environ. Microbiol. 74: 2534-2536 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»