This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Musovic, S.
Right arrow Articles by Sørensen, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Musovic, S.
Right arrow Articles by Sørensen, S. J.
Agricola
Right arrow Articles by Musovic, S.
Right arrow Articles by Sørensen, S. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2006, p. 6687-6692, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00013-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cultivation-Independent Examination of Horizontal Transfer and Host Range of an IncP-1 Plasmid among Gram-Positive and Gram-Negative Bacteria Indigenous to the Barley Rhizosphere

Sanin Musovic,1 Gunnar Oregaard,1 Niels Kroer,2 and Søren J. Sørensen1*

University of Copenhagen, Institute of Biology, Department of Microbiology, Sølvgade 83H, 1307K Copenhagen K, Denmark,1 National Environmental Research Institute, Department of Environmental Chemistry and Microbiology, Frederiksborgvej 399, 4000 Roskilde, Denmark2

Received 3 January 2006/ Accepted 24 July 2006

The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16S rRNA genes from the sorted cells. The host range of the pKJK10 plasmid was exceptionally broad, as it included not only bacteria belonging to the alpha, beta, and gamma subclasses of the Proteobacteria, but also Arthrobacter sp., a gram-positive member of the Actinobacteria. The transfer frequency (transconjugants per donor) from the Pseudomonas putida donor to the indigenous bacteria was 7.03 x 10–2 ± 3.84 x 10–2. This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ.

* Corresponding author. Mailing address: University of Copenhagen, Institute of Biology, Dept. of Microbiology, Sølvgade 83H, 1307K Copenhagen K, Denmark. Phone: 45 35 32 20 53. Fax: 45 35 32 20 40. E-mail: sjs{at}bi.ku.dk.

Applied and Environmental Microbiology, October 2006, p. 6687-6692, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00013-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Norman, A., Hansen, L. H., Sorensen, S. J. (2009). Conjugative plasmids: vessels of the communal gene pool. Phil Trans R Soc B 364: 2275-2289 [Abstract] [Full Text]  
  • Bahl, M. I., Hansen, L. H., Licht, T. R., Sorensen, S. J. (2007). Conjugative Transfer Facilitates Stable Maintenance of IncP-1 Plasmid pKJK5 in Escherichia coli Cells Colonizing the Gastrointestinal Tract of the Germfree Rat. Appl. Environ. Microbiol. 73: 341-343 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»