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Applied and Environmental Microbiology, October 2006, p. 6833-6836, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.01446-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Establishment of a Gene Expression System in Ochrobactrum anthropi

Mohamed N. Seleem,1 Mohammed Ali,2 Stephen M. Boyle,1 Biswarup Mukhopadhyay,3 Sharon G. Witonsky,1 Gerhardt G. Schurig,1 and Nammalwar Sriranganathan1*

Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine,1 Virginia Bioinformatics Institute, Virginia Polytechnic and State University, Blacksburg, Virginia,3 Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt2

Received 22 June 2006/ Accepted 26 July 2006

Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.

* Corresponding author. Mailing address: Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic and State University, 1410 Prices Fork Rd., Blacksburg, VA 24060. Phone: (540) 231-7171. Fax: (540) 231-3426. E-mail: nathans{at}vt.edu.

Applied and Environmental Microbiology, October 2006, p. 6833-6836, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.01446-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Doi, Y., Takaya, N., Takizawa, N. (2009). Novel Denitrifying Bacterium Ochrobactrum anthropi YD50.2 Tolerates High Levels of Reactive Nitrogen Oxides. Appl. Environ. Microbiol. 75: 5186-5194 [Abstract] [Full Text]  


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