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Applied and Environmental Microbiology, October 2006, p. 6845-6850, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00807-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Abundance and Population Structure of Ammonia-Oxidizing Bacteria That Inhabit Canal Sediments Receiving Effluents from Municipal Wastewater Treatment Plants{dagger}

Hidetoshi Urakawa,1,2* Hideaki Maki,1 Sumiko Kawabata,1 Taketomo Fujiwara,3 Haruo Ando,4 Toshio Kawai,4 Takehiko Hiwatari,1 Kunio Kohata,1 and Masataka Watanabe1,5

National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan,1 Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan,2 Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan,3 The Tokyo Metropolitan Research Institute for Environmental Protection, 1-7-5 Shinsuna, Koto, Tokyo 136-0075, Japan,4 Faculty of Environmental Information, Keio University, 5322 Endo, Fujisawa, Kanagawa 252-8520, Japan5

Received 6 April 2006/ Accepted 7 August 2006

A polyphasic, culture-independent study was conducted to investigate the abundance and population structure of ammonia-oxidizing bacteria (AOB) in canal sediments receiving wastewater discharge. The abundance of AOB ranged from 0.2 to 1.9% and 1.6 to 5.7% of the total bacterial fraction by real-time PCR and immunofluorescence staining, respectively. Clone analysis and restriction endonuclease analysis revealed that the AOB communities influenced by the wastewater discharge were dominated by Nitrosomonas, were similar to each other, and were less diverse than the communities outside of the immediate discharge zone.

* Corresponding author. Mailing address: Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan. Phone: (81) 3 5351 6854. Fax: (81) 3 5351 6820. E-mail: urakawa{at}ori.u-tokyo.ac.jp.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, October 2006, p. 6845-6850, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00807-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Urakawa, H., Tajima, Y., Numata, Y., Tsuneda, S. (2008). Low Temperature Decreases the Phylogenetic Diversity of Ammonia-Oxidizing Archaea and Bacteria in Aquarium Biofiltration Systems. Appl. Environ. Microbiol. 74: 894-900 [Abstract] [Full Text]  


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