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Applied and Environmental Microbiology, November 2006, p. 7401-7405, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01611-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Construction of a Reporter Vector for the Analysis of Bifidobacterium longum Promoters{triangledown}

A. Klijn,{dagger} D. Moine, M. Delley, A. Mercenier, F. Arigoni, and R. D. Pridmore*

Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland

Received 12 July 2006/ Accepted 29 August 2006

In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.

* Corresponding author. Mailing address: Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland. Phone: 41-021-785-8654. Fax: 41-021-785-8544. E-mail: raymond-david.pridmore{at}rdls.nestle.com.

{triangledown} Published ahead of print on 22 September 2006.

{dagger} Present address: Nestlé UK, Haxby Road, York YO91 1XY, England.

Applied and Environmental Microbiology, November 2006, p. 7401-7405, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01611-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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