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Applied and Environmental Microbiology, November 2006, p. 7413-7417, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01162-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Degradation of Fluorobenzene by Rhizobiales Strain F11 via ortho Cleavage of 4-Fluorocatechol and Catechol{triangledown}

Maria F. Carvalho,1,§ Maria Isabel M. Ferreira,2,§ Irina S. Moreira,1 Paula M. L. Castro,1* and Dick B. Janssen2

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal,1 Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL-9747 AG Groningen, The Netherlands2

Received 19 May 2006/ Accepted 5 September 2006

The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.

* Corresponding author. Mailing address: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. Phone: 351 225580059. Fax: 351 225090351. E-mail: plc{at}esb.ucp.pt.

{triangledown} Published ahead of print on 15 September 2006.

§ M.F.C. and M.I.M.F. contributed equally to this work.

Applied and Environmental Microbiology, November 2006, p. 7413-7417, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01162-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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