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Applied and Environmental Microbiology, December 2006, p. 7894-7896, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.00965-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices{triangledown}

Silvia Bofill-Mas,1 Nestor Albinana-Gimenez,1 Pilar Clemente-Casares,1 Ayalkibet Hundesa,1 Jesus Rodriguez-Manzano,1 Annika Allard,3 Miquel Calvo,2 and Rosina Girones1*

Department of Microbiology,1 Department of Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 645, Barcelona 08028, Spain,2 Department of Clinical Virology, Umeå University Hospital, S-901 85 Umeå, Sweden3

Received 24 April 2006/ Accepted 2 October 2006

Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

Corresponding author. Mailing address: Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 645, Barcelona 08028, Spain. Phone: 34 93 402 14 83. Fax: 34 93 4039047. E-mail: rgirones{at}ub.edu.

{triangledown} Published ahead of print on 6 October 2006.

Applied and Environmental Microbiology, December 2006, p. 7894-7896, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.00965-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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