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Applied and Environmental Microbiology, December 2006, p. 7897-7901, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01526-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Synergistic Inhibition of Microbial Sulfide Production by Combinations of the Metabolic Inhibitor Nitrite and Biocides{triangledown}

E. Anne Greene,1 Veronique Brunelle,1 Gary E. Jenneman,2 and Gerrit Voordouw1*

Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada,1 ConocoPhillips, 224 GB, Bartlesville, Oklahoma 740042

Received 1 July 2006/ Accepted 18 September 2006

MICs of six broad-spectrum biocides and two specific metabolic inhibitors and fractional inhibitory concentration indexes (FICIs) for controlling a sulfide-producing consortium were determined. Nitrite was synergistic (FICI < 1) with all but one biocide due to its specific inhibition of dissimilatory sulfite reductase. Hence, combining nitrite with biocides allows more efficient and cost-effective control of sulfate-reducing bacteria.


* Corresponding author. Mailing address: Department of Biological Sciences, University of Calgary, 2500 University Dr. NW, Calgary, Alberta T2N 1N4, Canada. Phone: (403) 220-6388. Fax: (403) 289-9311. E-mail: voordouw{at}ucalgary.ca.

{triangledown} Published ahead of print on 22 September 2006.


Applied and Environmental Microbiology, December 2006, p. 7897-7901, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01526-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.







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