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Applied and Environmental Microbiology, February 2006, p. 1702-1704, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1702-1704.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare

Lotta-Riina Suomalainen, Hilkka Reunanen, Ritva Ijäs, E. Tellervo Valtonen, and Marja Tiirola*

Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland

Received 11 August 2005/ Accepted 23 November 2005

Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.

* Corresponding author. Mailing address: Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland. Phone: 358142602334. Fax: 358142602321. E-mail: mtiirola{at}jyu.fi.

Applied and Environmental Microbiology, February 2006, p. 1702-1704, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1702-1704.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sekar, R., Kaczmarsky, L. T., Richardson, L. L. (2009). Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals. Appl. Environ. Microbiol. 75: 2581-2584 [Abstract] [Full Text]  


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