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Applied and Environmental Microbiology, March 2006, p. 2134-2140, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2134-2140.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Minimization of Glycerol Production during the High-Performance Fed-Batch Ethanolic Fermentation Process in Saccharomyces cerevisiae, Using a Metabolic Model as a Prediction Tool{dagger}

Carine Bideaux,{ddagger} Sandrine Alfenore,{ddagger} Xavier Cameleyre, Carole Molina-Jouve, Jean-Louis Uribelarrea, and Stéphane E. Guillouet*

Biotechnology and Bioprocess Laboratory, UMR-CNRS 5504, UMR-INRA 792, Département de Génie Biochimique et Alimentaire, Institut National des Sciences Appliquées, 135 Avenue de Rangueil, 31077 Toulouse Cedex, France

Received 21 July 2005/ Accepted 29 November 2005

On the basis of knowledge of the biological role of glycerol in the redox balance of Saccharomyces cerevisiae, a fermentation strategy was defined to reduce the surplus formation of NADH, responsible for glycerol synthesis. A metabolic model was used to predict the operating conditions that would reduce glycerol production during ethanol fermentation. Experimental validation of the simulation results was done by monitoring the inlet substrate feeding during fed-batch S. cerevisiae cultivation in order to maintain the respiratory quotient (RQ) (defined as the CO2 production to O2 consumption ratio) value between 4 and 5. Compared to previous fermentations without glucose monitoring, the final glycerol concentration was successfully decreased. Although RQ-controlled fermentation led to a lower maximum specific ethanol production rate, it was possible to reach a high level of ethanol production: 85 g · liter–1 with 1.7 g · liter–1 glycerol in 30 h. We showed here that by using a metabolic model as a tool in prediction, it was possible to reduce glycerol production in a very high-performance ethanolic fermentation process.

* Corresponding author. Mailing address: Biotechnology and Bioprocess Laboratory, UMR-CNRS 5504, UMR-INRA 792, Département de Génie Biochimique et Alimentaire, Institut National des Sciences Appliquées, 135 Avenue de Rangueil, 31077 Toulouse Cedex, France. Phone: (33) 5 61 55 94 47. Fax: (33) 5 61 55 94 00. E-mail: guillouet{at}insa-toulouse.fr.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} These two authors worked equally on this project.

Applied and Environmental Microbiology, March 2006, p. 2134-2140, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2134-2140.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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