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 Previous Article

Applied and Environmental Microbiology, March 2006, p. 2290-2293, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2290-2293.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Transformation of Rhizobia with Broad-Host-Range Plasmids by Using a Freeze-Thaw Method

Eva Vincze1* and Steve Bowra2

Department of Genetics and Biotechnology, Research Centre Flakkebjerg, Danish Institute of Agricultural Sciences, Forsøgsvej 1, DK-4200 Slagelse, Denmark,1 Phytatec (UK) Ltd., Plas Gogerddan, Aberystwyth, Wales SY23 3EB, United Kingdom2

Received 21 July 2005/ Accepted 11 January 2006

Several species of rhizobia were successfully transformed with broad-host-range plasmids of different replicons by using a modified freeze-thaw method. A generic binary vector (pPZP211) was maintained in Mesorhizobium loti without selection and stably inherited during nodulation. The method could extend the potential of rhizobia as a vehicle for plant transformation.

* Corresponding author. Mailing address: Department of Genetics and Biotechnology, Research Centre Flakkebjerg, Danish Institute of Agricultural Sciences, Forsøgsvej 1, DK-4200 Slagelse, Denmark. Phone: 45 89 99 37 79. Fax: 45 89 99 35 01. E-mail: eva.vincze{at}agrsci.dk.

Applied and Environmental Microbiology, March 2006, p. 2290-2293, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2290-2293.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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