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Applied and Environmental Microbiology, May 2006, p. 3154-3160, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3154-3160.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Isolation and Sequencing of a Temperate Transducing Phage for Pasteurella multocida

Susana Campoy,1,{dagger} Jesús Aranda,2,{dagger} Gerard Àlvarez,2 Jordi Barbé,1,2 and Montserrat Llagostera1,2*

Centre de Recerca en Sanitat Animal (CReSA),1 Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain2

Received 9 November 2005/ Accepted 20 February 2006

A temperate bacteriophage (F108) has been isolated through mitomycin C induction of a Pasteurella multocida serogroup A strain. F108 has a typical morphology of the family Myoviridae, presenting a hexagonal head and a long contractile tail. F108 is able to infect all P. multocida serogroup A strains tested but not those belonging to other serotypes. Bacteriophage F108, the first P. multocida phage sequenced so far, presents a 30,505-bp double-stranded DNA genome with cohesive ends (CTTCCTCCCC cos site). The F108 genome shows the highest homology with those of Haemophilus influenzae HP1 and HP2 phages. Furthermore, an F108 prophage attachment site in the P. multocida chromosome has been established to be inside a gene encoding tRNALeu. By using several chromosomal markers that are spread along the P. multocida chromosome, it has been demonstrated that F108 is able to perform generalized transduction. This fact, together with the absence of pathogenic genes in the F108 genome, makes this bacteriophage a valuable tool for P. multocida genetic manipulation.

* Corresponding author. Mailing address: Universitat Autònoma de Barcelona, Departament de Genètica i Microbiologia, Unitat de Microbiologia, Edifici C, Bellaterra, 08193 Barcelona, Spain. Phone: 34 93 581 2615. Fax: 34 93 581 2387. E-mail: montserrat.llagostera{at}uab.es.

{dagger} J.A. and S.C. should be regarded as joint first authors of this work.

Applied and Environmental Microbiology, May 2006, p. 3154-3160, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3154-3160.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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