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Applied and Environmental Microbiology, May 2006, p. 3236-3244, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3236-3244.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Metal Reduction and Iron Biomineralization by a Psychrotolerant Fe(III)-Reducing Bacterium, Shewanella sp. Strain PV-4

Yul Roh,^1,2, Haichun Gao,^1,3, Hojatollah Vali,⁴ David W. Kennedy,⁵ Zamin K. Yang,¹ Weimin Gao,¹ Alice C. Dohnalkova,⁵ Raymond D. Stapleton,⁶ Ji-Won Moon,¹ Tommy J. Phelps,¹ James K. Fredrickson,⁵ and Jizhong Zhou^1,7*

Oak Ridge National Laboratory, Oak Ridge, Tennessee,¹ Chonnam National University, Gwangju, South Korea,² Center for Microbial Ecology, Michigan State University, East Lansing, Michigan,³ McGill University, Montreal, Quebec, Canada,⁴ Pacific Northwest National Laboratory, Richland, Washington,⁵ Merck & Co., Inc., Elkton, Virginia,⁶ Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019⁷

Received 9 May 2005/ Accepted 5 February 2006

A marine psychrotolerant, dissimilatory Fe(III)-reducing bacterium, Shewanella sp. strain PV-4, from the microbial mat at a hydrothermal vent of Loihi Seamount in the Pacific Ocean has been further characterized, with emphases on metal reduction and iron biomineralization. The strain is able to reduce metals such as Fe(III), Co(III), Cr(VI), Mn(IV), and U(VI) as electron acceptors while using lactate, formate, pyruvate, or hydrogen as an electron donor. Growth during iron reduction occurred over the pH range of 7.0 to 8.9, a sodium chloride range of 0.05 to 5%, and a temperature range of 0 to 37°C, with an optimum growth temperature of 18°C. Unlike mesophilic dissimilatory Fe(III)-reducing bacteria, which produce mostly superparamagnetic magnetite (<35 nm), this psychrotolerant bacterium produces well-formed single-domain magnetite (>35 nm) at temperatures from 18 to 37°C. The genome size of this strain is about 4.5 Mb. Strain PV-4 is sensitive to a variety of commonly used antibiotics except ampicillin and can acquire exogenous DNA (plasmid pCM157) through conjugation.

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* Corresponding author. Mailing address: Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019. Phone: (405) 325-6073. Fax: (405) 325-3442. E-mail: jzhou{at}ou.edu.

Y.R. and H.G. contributed equally to this work.

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Applied and Environmental Microbiology, May 2006, p. 3236-3244, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3236-3244.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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