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Applied and Environmental Microbiology, May 2006, p. 3367-3374, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3367-3374.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Modeling for Gellan Gum Production by Sphingomonas paucimobilis ATCC 31461 in a Simplified Medium

Xia Wang, Ping Xu,* Yong Yuan, Changlong Liu, Dezhong Zhang, Zhengting Yang, Chunyu Yang, and Cuiqing Ma

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China

Received 23 November 2005/ Accepted 2 March 2006

Gellan gum production was carried out by Sphingomonas paucimobilis ATCC 31461 in a simplified medium with a short incubation time, and a kinetic model for understanding, controlling, and optimizing the fermentation process was proposed. The results revealed that glucose was the best carbon source and that the optimal concentration was 30 g liter–1. As for the fermenting parameters, considerably large amounts of gellan gum were yielded by an 8-h-old culture and a 4% inoculum at 200 rpm on a rotary shaker. Under the optimized conditions, the maximum level of gellan gum (14.75 g liter–1) and the highest conversion efficiency (49.17%) were obtained in a 30-liter fermentor in batch fermentation. Logistic and Luedeking-Piret models were confirmed to provide a good description of gellan gum fermentation, which gave some support for the study of gellan gum fermentation kinetics. Additionally, this study is the first demonstration that gellan gum production is largely growth associated by analysis of kinetics in its batch fermentation process. Based on model prediction, higher gellan gum production (17.71 g liter–1) and higher conversion efficiency (57.12%) were obtained in fed-batch fermentation at the same total glucose concentration (30 g liter–1).

* Corresponding author. Mailing address: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China. Phone: 86-531-88564003. Fax: 86-531-88567250. E-mail: pingxu{at}sdu.edu.cn.

Applied and Environmental Microbiology, May 2006, p. 3367-3374, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3367-3374.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wang, X., Gai, Z., Yu, B., Feng, J., Xu, C., Yuan, Y., Lin, Z., Xu, P. (2007). Degradation of Carbazole by Microbial Cells Immobilized in Magnetic Gellan Gum Gel Beads. Appl. Environ. Microbiol. 73: 6421-6428 [Abstract] [Full Text]  


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