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Applied and Environmental Microbiology, May 2006, p. 3785-3787, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3785-3787.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Single-Nucleotide-Polymorphism-Specific PCR for Quantification and Discrimination of Chlamydia pneumoniae Genotypes by Use of a "Locked" Nucleic Acid

Jan Rupp, Werner Solbach, and Jens Gieffers*

Institute of Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

Received 27 December 2005/ Accepted 14 March 2006

Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany. Phone: 49/451/5004409. Fax: 49/451/5002808. E-mail: Jens.Gieffers{at}hygiene.ukl.mu-luebeck.de.

Applied and Environmental Microbiology, May 2006, p. 3785-3787, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3785-3787.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Levin, J. D., Fiala, D., Samala, M. F., Kahn, J. D., Peterson, R. J. (2006). Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers. Nucleic Acids Res 34: e142-e142 [Abstract] [Full Text]  


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