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Applied and Environmental Microbiology, June 2006, p. 4192-4199, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02889-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analysis of Quantitative Interactions between Two Species of Arbuscular Mycorrhizal Fungi, Glomus mosseae and G. intraradices, by Real-Time PCR

Noam Alkan,1 Vijay Gadkar,2 Oded Yarden,3 and Yoram Kapulnik1*

Department of Agronomy & Natural Resources, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel,1 Microbial Ecology Program, Division of Biological Sciences, 32 Campus Dr. 4824, Health Sciences Bldg., University of Montana, Missoula, Montana 59812,2 Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76 100, Israel3

Received 8 December 2005/ Accepted 4 April 2006

Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs, known to play an important role in ecological processes. Conventional light microscopy is the most common method used to detect their presence in planta, but this method fails to discern the presence of multiple AMF species and is not quantitative. These two factors are critically important in ecological studies, where the symbiotic contribution of each isolate needs to be defined. This paper describes the use of quantitative real-time PCR (qRT-PCR) as a detection system to address this issue. We used two Glomus spp., namely, G. intraradices and G. mosseae, to show that it is possible to study the interactions between these two isolates during the cocolonization of a single root system. Three different physiological studies were set up to assess how the interactions affected the occupancy of these fungi intraradically on a temporal basis. These treatments included saline and phosphorus stress, spatial distribution in the root zone, and preference for a particular host. qRT-PCR could prove a valuable tool in the area of AMF field ecology, where such data are critically important for defining the role of each species in the community structure.

* Corresponding author. Mailing address: Department of Agronomy & Natural Resources, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50 250, Israel. Phone: 972-3-968-3900. Fax: 972-3-966-9642. E-mail: Kapulnik{at}volcani.agri.gov.il.

Applied and Environmental Microbiology, June 2006, p. 4192-4199, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02889-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gutjahr, C., Banba, M., Croset, V., An, K., Miyao, A., An, G., Hirochika, H., Imaizumi-Anraku, H., Paszkowski, U. (2008). Arbuscular Mycorrhiza-Specific Signaling in Rice Transcends the Common Symbiosis Signaling Pathway. Plant Cell 20: 2989-3005 [Abstract] [Full Text]  


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