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Applied and Environmental Microbiology, June 2006, p. 4360-4369, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00132-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

New Lineage of Filamentous, Spore-Forming, Gram-Positive Bacteria from Soil

Linda Cavaletti,1 Paolo Monciardini,1 Ruggiero Bamonte,1 Peter Schumann,2 Manfred Rohde,3 Margherita Sosio,1 and Stefano Donadio1,4*

Vicuron Pharmaceuticals, 21040 Gerenzano, Italy,1 DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-38124 Braunschweig, Germany,2 GBF-Gesellschaft für Biotechnologische Forschung GmbH, D-38124 Braunschweig, Germany,3 KtedoGen, 21046 Malnate, Italy4

Received 18 January 2006/ Accepted 29 March 2006

A novel bacterial strain that was isolated from an Italian soil and was designated SOSP1-21T forms branched mycelia in solid and liquid media and has a filamentous morphology similar to that of some genera belonging to the Actinobacteria. Electron microscopy showed that this organism has a grape-like appearance, resulting from interlacing of spores originating from sporophoric hyphae. Ten strains that are morphologically related to SOSP1-21T were recovered from soil. Phylogenetic analyses of 16S rRNA gene segments confirmed the relatedness of these strains to SOSP1-21T and indicated that the newly isolated strains form separate clades in a deeply branching lineage. The closest matches for the 16S rRNA sequences of all the strains (around 79% identity) were matches with representatives of the Chloroflexi, although the affiliation with this division was not supported by high bootstrap values. The strains are mesophilic aerobic heterotrophs and are also capable of growing under microaerophilic conditions. They all stain gram positive. Strain SOSP1-21T contains ornithine, alanine, glutamic acid, serine, and glycine as the peptidoglycan amino acids. In addition, an unusual level of C16:1 2OH (30%) was found in the cellular fatty acids. The G+C content of SOSP1-21T genomic DNA is 53.9%, and MK-9(H2) was the only menaquinone detected. All these data suggest that SOSP1-21T and the related strains may constitute a new division of filamentous, spore-forming, gram-positive bacteria. We propose the name Ktedobacter racemifer gen. nov., sp. nov. for strain SOSP1-21T.

* Corresponding author. Mailing address: KtedoGen, via Cav. Brusa 43, 21046 Malnate, Italy. Phone: 393403718661. Fax: 390332428812. E-mail: stefano_donadio{at}libero.it.

Applied and Environmental Microbiology, June 2006, p. 4360-4369, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00132-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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