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Applied and Environmental Microbiology, June 2006, p. 4492-4496, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02543-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Overproduction of Wild-Type and Bioengineered Derivatives of the Lantibiotic Lacticin 3147

Paul D. Cotter,1 Lorraine A. Draper,1 Elaine M. Lawton,1 Olivia McAuliffe,2 Colin Hill,1,3* and R. Paul Ross2,3

Department of Microbiology, University College Cork, Cork, Ireland,1 Teagasc Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Ireland,2 Alimentary Pharmabiotic Centre, Cork, Ireland3

Received 28 October 2005/ Accepted 26 March 2006

Lacticin 3147 is a broad-spectrum two-peptide lantibiotic whose genetic determinants are located on two divergent operons on the lactococcal plasmid pMRC01. Here we introduce each of 14 subclones, containing different combinations of lacticin 3147 genes, into MG1363 (pMRC01) and determine that a number of them can facilitate overproduction of the lantibiotic. Based on these studies it is apparent that while the provision of additional copies of genes encoding the biosynthetic/production machinery and the regulator LtnR is a requirement for high-level overproduction, the presence of additional copies of the structural genes (i.e., ltnA1A2) is not.

* Corresponding author. Mailing address: Department of Microbiology, University College Cork, Cork, Ireland. Phone: 353-21-4901373. Fax: 353-21-4903101. E-mail: c.hill{at}ucc.ie.

Applied and Environmental Microbiology, June 2006, p. 4492-4496, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02543-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Bottiger, T., Schneider, T., Martinez, B., Sahl, H.-G., Wiedemann, I. (2009). Influence of Ca2+ Ions on the Activity of Lantibiotics Containing a Mersacidin-Like Lipid II Binding Motif. Appl. Environ. Microbiol. 75: 4427-4434 [Abstract] [Full Text]  
  • Kuipers, A., Meijer-Wierenga, J., Rink, R., Kluskens, L. D., Moll, G. N. (2008). Mechanistic Dissection of the Enzyme Complexes Involved in Biosynthesis of Lacticin 3147 and Nisin. Appl. Environ. Microbiol. 74: 6591-6597 [Abstract] [Full Text]  
  • Guinane, C. M., Cotter, P. D., Lawton, E. M., Hill, C., Ross, R. P. (2007). Insertional Mutagenesis To Generate Lantibiotic Resistance in Lactococcus lactis. Appl. Environ. Microbiol. 73: 4677-4680 [Abstract] [Full Text]  


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