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Applied and Environmental Microbiology, July 2006, p. 4619-4626, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.02919-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of an Abscisic Acid Gene Cluster in the Grey Mold Botrytis cinerea

Verena Siewers,1,{dagger} Leonie Kokkelink,1 Jørn Smedsgaard,2 and Paul Tudzynski1*

Westfälische Wilhelms-Universität, Institut für Botanik, Schlossgarten 3, D-48149 Münster, Germany,1 Bio-Centrum DTU, Building 221, Søltofts Plads, DK-2800 Lyngby, Denmark2

Received 12 December 2005/ Accepted 26 April 2006

Like several other phytopathogenic fungi, the ascomycete Botrytis cinerea is known to produce the plant hormone abscisic acid (ABA) in axenic culture. Recently, bcaba1, the first fungal gene involved in ABA biosynthesis, was identified. Neighborhood analysis of bcaba1 revealed three further candidate genes of this pathway: a putative P450 monooxygenase-encoding gene (bcaba2), an open reading frame without significant similarities (bcaba3), and a gene probably coding for a short-chain dehydrogenase/reductase (bcaba4). Targeted inactivation of the genes proved the involvement of BcABA2 and BcABA3 in ABA biosynthesis and suggested a contribution of BcABA4. The close linkage of at least three ABA biosynthetic genes is strong evidence for the presence of an abscisic acid gene cluster in B. cinerea.

* Corresponding author. Mailing address: Institut für Botanik, Westf. Wilhelms-Universität Münster, Schlossgarten 3, 48149 Münster, Germany. Phone: 49-251-83 24998. Fax: 49-251-83 21601. E-mail: tudzyns{at}uni-muenster.de.

{dagger} Present address: Bio-Centrum DTU, Building 223, Søltofts Plads, DK-2800 Lyngby, Denmark.

Applied and Environmental Microbiology, July 2006, p. 4619-4626, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.02919-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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