This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Finger, S. A.
Right arrow Articles by Gilman, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Finger, S. A.
Right arrow Articles by Gilman, R. H.
Agricola
Right arrow Articles by Finger, S. A.
Right arrow Articles by Gilman, R. H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2006, p. 4713-4716, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00894-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Effectiveness of Enterobacterial Repetitive Intergenic Consensus PCR and Random Amplified Polymorphic DNA Fingerprinting for Helicobacter pylori Strain Differentiation

S. Alison Finger,1,4 Billie Velapatiño,1,2 Margaret Kosek,3 Livia Santivañez,1,2,5 Daiva Dailidiene,5 Willi Quino,1 Jacqueline Balqui,1,2 Phabiola Herrera,1,2 Douglas E. Berg,5* and Robert H. Gilman1,2,3*

Departamento de Microbiología, Universidad Peruana Cayetano Heredia, Lima, Peru,1 Asociación Benéfica PRISMA, Lima, Peru,2 Department of International Health, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland,3 Washington University School of Medicine, St. Louis, Missouri,4 Department of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri5

Received 16 April 2006/ Accepted 20 April 2006

We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed Helicobacter pylori infection in Peruvian shantytown residents. H. pylori isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each participant were analyzed by ERIC-PCR and by RAPD tests with four 10-nucleotide primers (one primer per reaction). There was 94% agreement between the ERIC and RAPD profiles in classifying sets of isolates as uniform versus closely related but not identical versus probably unrelated, indicating a high kappa statistic of 0.8942. Subtle differences in related ERIC or RAPD patterns likely reflect gene transfer between strains, recombination, and/or mutation, whereas markedly different patterns reflect infection by unrelated strains. At least half of infected shantytown residents seemed to carry more than one H. pylori strain, although in 19 of 31 persons, the strains were closely related. Three RAPD tests, each with a different primer, were needed to achieve the sensitivity of one ERIC test. ERIC-PCR constitutes a resource- and time-efficient method for H. pylori strain differentiation.

* Corresponding author. Mailing address for D. E. Berg: Department of Molecular Microbiology, Campus Box 8230, Washington University Medical School, 4940 Parkview Place, St. Louis, MO 63110. Phone: (314) 362-2772. Fax: (314) 362-1232. E-mail: berg{at}borcim.wustl.edu. Mailing address for R. H. Gilman: The Johns Hopkins School of Public Health, Department of International Health, 615 N. Wolfe Street, Rm. W3501, Baltimore, MD 21205. Phone: (410) 955-6964. Fax: (410) 502-6733. E-mail: gilmanbob{at}yahoo.com.

Applied and Environmental Microbiology, July 2006, p. 4713-4716, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00894-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»