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Applied and Environmental Microbiology, July 2006, p. 4871-4877, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00375-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Amber Vanden Wymelenberg,1 Grzegorz Sabat,2 Michael Mozuch,3 Philip J. Kersten,3 Dan Cullen,3* and Robert A. Blanchette4

Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706,1 Genetics and Biotechnology Center, University of Wisconsin, Madison, Wisconsin 53706,2 USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin 53726,3 Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 551084

Received 15 February 2006/ Accepted 12 May 2006

The white rot basidiomycete Phanerochaete chrysosporium produces an array of nonspecific extracellular enzymes thought to be involved in lignin degradation, including lignin peroxidases, manganese peroxidases, and the H2O2-generating copper radical oxidase, glyoxal oxidase (GLX). Preliminary analysis of the P. chrysosporium draft genome had identified six sequences with significant similarity to GLX and designated cro1 through cro6. The predicted mature protein sequences diverge substantially from one another, but the residues coordinating copper and constituting the radical redox site are conserved. Transcript profiles, microscopic examination, and lignin analysis of inoculated thin wood sections are consistent with differential regulation as decay advances. The cro2-encoded protein was detected by liquid chromatography-tandem mass spectrometry in defined medium. The cro2 cDNA was successfully expressed in Aspergillus nidulans under the control of the A. niger glucoamylase promoter and secretion signal. The recombinant CRO2 protein had a substantially different substrate preference than GLX. The role of structurally and functionally diverse cro genes in lignocellulose degradation remains to be established.

* Corresponding author. Mailing address: Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53726. Phone: (608) 231-9468. Fax: (608) 231-9262. E-mail: dcullen{at}facstaff.wisc.edu.

Applied and Environmental Microbiology, July 2006, p. 4871-4877, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00375-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vanden Wymelenberg, A., Gaskell, J., Mozuch, M., Kersten, P., Sabat, G., Martinez, D., Cullen, D. (2009). Transcriptome and Secretome Analyses of Phanerochaete chrysosporium Reveal Complex Patterns of Gene Expression. Appl. Environ. Microbiol. 75: 4058-4068 [Abstract] [Full Text]  


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