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Applied and Environmental Microbiology, July 2006, p. 5086-5088, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00157-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

In Vivo Transduction of an Stx-Encoding Phage in Ruminants

Nancy A. Cornick,1* Amy F. Helgerson,1 Volker Mai,2 Jennifer M. Ritchie,3,{dagger} and David W. K. Acheson2

Department of Veterinary Microbiology and Preventative Medicine, Iowa State University, Ames, Iowa,1 Department of Epidemiology and Preventative Medicine, University of Maryland Medical School, Baltimore, Maryland,2 New England Medical Center, Boston, Massachusetts3

Received 20 January 2006/ Accepted 23 April 2006

We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.

* Corresponding author. Mailing address: 2130 Vet. Med. Bldg., Iowa State University, Ames, IA 50010. Phone: (515) 294-6499. Fax: (515) 294-8500. E-mail: ncornick{at}iastate.edu.

{dagger} Present address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Mass.

Applied and Environmental Microbiology, July 2006, p. 5086-5088, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00157-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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