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Applied and Environmental Microbiology, August 2006, p. 5618-5622, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00029-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Direct Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef

Cecilia Fontana,1,2 Pier Sandro Cocconcelli,2,3 and Graciela Vignolo1*

Centro de Referencia para Lactobacilos (CERELA), CONICET, Chacabuco 145, T4000ILC Tucumán, Argentina,1 Centro Ricerche Biotecnologiche, Università Cattolica del Sacro Cuore, via Milano 24, 26100 Cremona, Italy,2 Istituto di Microbiologia, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29100 Piacenza, Italy3

Received 5 January 2006/ Accepted 16 May 2006

Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8°C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche.

* Corresponding author. Mailing address: Centro de Referencia para Lactobacilos, CERELA (CONICET), Chacabuco 145, T4000ILC Tucumán, Argentina. Phone: 54-381-4311720. Fax: 54-381-4005600. E-mail: vignolo{at}cerela.org.ar.

Applied and Environmental Microbiology, August 2006, p. 5618-5622, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00029-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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