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Applied and Environmental Microbiology, August 2006, p. 5637-5642, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00739-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression

Maximilian Schlicht, Christian Berens, Janko Daam, and Wolfgang Hillen*

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

Received 30 March 2006/ Accepted 19 May 2006

The insertion element InsTip{alpha} was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany. Phone: 49-9131-85-28081. Fax: 49-9131-85-28082. E-mail: whillen{at}biologie.uni-erlangen.de.

Applied and Environmental Microbiology, August 2006, p. 5637-5642, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00739-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Klotzsche, M., Goeke, D., Berens, C., Hillen, W. (2007). Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site. Nucleic Acids Res 35: 3945-3952 [Abstract] [Full Text]  


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