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Applied and Environmental Microbiology, September 2006, p. 5766-5776, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00431-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Tick-Borne Rickettsial Pathogens in Ticks and Small Mammals in Korea

Chul-Min Kim,1 Ying-Hua Yi,1 Do-Hyeon Yu,1 Mi-Jin Lee,1 Mae-Rim Cho,1 Atul R. Desai,1 Smriti Shringi,1 Terry A. Klein,2 Heung-Chul Kim,3 Jin-Won Song,4 Luck-Ju Baek,4 Sung-Tae Chong,3 Monica L. O'Guinn,5 John S. Lee,5 In-Yong Lee,6 Jin-Ho Park,1 Janet Foley,7 and Joon-Seok Chae1*

Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Korea,1 Force Health Protection, 18th Medical Command, Unit 15821, Box 754, APO AP 96205-5281,2 5th Medical Detachment, 168th Medical Battalion (AS), 18th Medical Command, Unit 15247, APO AP 96205-5247,3 Department of Microbiology, College of Medicine, Korea University, 126-1, 5 Ka, Anam-Dong, Sungbuk-Gu, Seoul 136-705, Korea,4 Department of Virology, 1425 Porter Street, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011,5 Department of Parasitology, College of Medicine, Yonsei University, Seoul 120-749, Korea,6 Center for Vector-Borne Diseases, School of Veterinary Medicine, University of California, Davis, California 956167

Received 21 February 2006/ Accepted 16 June 2006

In order to investigate the prevalence of tick-borne infectious agents among ticks, ticks comprising five species from two genera (Hemaphysalis spp. and Ixodes spp.) were screened using molecular techniques. Ticks (3,135) were collected from small wild-caught mammals or by dragging/flagging in the Republic of Korea (ROK) and were pooled into a total of 1,638 samples (1 to 27 ticks per pool). From the 1,638 tick samples, species-specific fragments of Anaplasma phagocytophilum (1 sample), Anaplasma platys (52 samples), Ehrlichia chaffeensis (29 samples), Ehrlichia ewingii (2 samples), Ehrlichia canis (18 samples), and Rickettsia rickettsii (28 samples) were amplified by PCR assay. Twenty-one pooled and individual tick samples had mixed infections of two (15 samples) or three (6 samples) pathogens. In addition, 424 spleen samples from small captured mammals (389 rodents, 33 insectivores, and 2 weasels) were screened for selected zoonotic pathogens. Species-specific DNA fragments of A. phagocytophilum (110 samples), A. platys (68 samples), E. chaffeensis (8 samples), E. ewingii (26 samples), E. canis (51 samples), and Rickettsia sp. (22 samples) were amplified by PCR assay. One hundred thirty small mammals had single infections, while 4, 14, and 21 striped field mice (Apodemus agrarius) had mixed infections of four, three, and two pathogens, respectively. Phylogenetic analysis based on nucleotide sequence comparison also revealed that Korean strains of E. chaffeensis clustered closely with those from China and the United States, while the Rickettsia (rOmpA) sequences clustered within a clade together with a Chinese strain. These results suggest that these agents should be considered in differential diagnosis while examining cases of acute febrile illnesses in humans as well as animals in the ROK.

* Corresponding author. Present address: Center for Vector-Borne Diseases, Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California—Davis, One Shields Ave., Davis, CA 95616. Phone: (530) 754-9740. Fax: (530) 754-4837. E-mail: jschae{at}ucdavis.edu.

Applied and Environmental Microbiology, September 2006, p. 5766-5776, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00431-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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