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Applied and Environmental Microbiology, September 2006, p. 6142-6151, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00138-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of Salmonella enterica Serovar Typhimurium LT2 and Non-LT2 Salmonella Genomic Sequences, and Genotyping of Salmonellae by Using PCR{dagger}

Hyun-Joong Kim, Si-Hong Park, and Hae-Yeong Kim*

Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea

Received 18 January 2006/ Accepted 27 June 2006

Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Salmonella bongori (V), and were clustered into 17 groups by their comparison patterns. A total of 38 primer pairs were constructed to represent each of the 17 groups, and PCR was performed with various Salmonella subspecies including Salmonella enterica subsp. I, II, IIIa, IIIb, IV, VI, and V to evaluate a comprehensive DNA-based scheme for identification of Salmonella subspecies and the major disease-causing Salmonella serovars. Analysis of PCR results showed that Salmonella enterica subsp. I was critically divided from other subspecies, and Salmonella strains belonging to S. enterica subsp. I were clustered based on their serovars. In addition, genotypic relationships within S. enterica subsp. I by PCR results were investigated. Also, Salmonella signature genes, Salmonella enterica serovar Typhimurium signature genes, and Salmonella enterica subsp. I signature genes were demonstrated based on their PCR results. The described PCR method suggests a rapid and convenient method for identification of Salmonella serovars that can be used by nonspecialized laboratories. Genome sequence comparison can be a useful tool in epidemiologic and taxonomic studies of Salmonella.

* Corresponding author. Mailing address: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea. Phone: 82 31 201 2660. Fax: 82 31 204 8116. E-mail: hykim{at}khu.ac.kr.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2006, p. 6142-6151, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00138-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Katribe, E., Bogomolnaya, L. M., Wingert, H., Andrews-Polymenis, H. (2009). Subspecies IIIa and IIIb Salmonellae Are Defective for Colonization of Murine Models of Salmonellosis Compared to Salmonella enterica subsp. I Serovar Typhimurium. J. Bacteriol. 191: 2843-2850 [Abstract] [Full Text]  


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