This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakanishi, Y. Articles by Benno, Y. Search for Related Content PubMed PubMed Citation Articles by Nakanishi, Y. Articles by Benno, Y. Agricola Articles by Nakanishi, Y. Articles by Benno, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2006, p. 6271-6276, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Increase in Terminal Restriction Fragments of Bacteroidetes-Derived 16S rRNA Genes after Administration of Short-Chain Fructooligosaccharides

Yusuke Nakanishi,^1, Koichiro Murashima,^2*, Hiroki Ohara,² Takahisa Suzuki,³ Hidenori Hayashi,⁴ Mitsuo Sakamoto,⁴ Tomoyuki Fukasawa,² Hidetoshi Kubota,² Akira Hosono,¹ Toshiaki Kono,² Shuichi Kaminogawa,¹ and Yoshimi Benno⁴

Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510,¹ Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1 Chiyoda, Sakado, Saimata 350-0289,² Pharmaceutical Research Department, Meiji Seika Kaisha, Ltd., 760 Morooka, Kohoku-Ku, Yokohama, Kanagawa 222-8567 and,³ Microbe Division, Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan⁴

Received 27 February 2006/ Accepted 22 June 2006

It is well known that short chain fructooligosaccharides (scFOS) modify intestinal microbiota in animals as well as in humans. Since most murine intestinal bacteria are still uncultured, it is difficult for a culturing method to detect changes in intestinal microbiota after scFOS administration in a mouse model. In this study, we sought markers of positive change in murine intestinal microbiota after scFOS administration using terminal restriction fragment length polymorphism (T-RFLP) analysis, which is a culture-independent method. The T-RFLP profiles showed that six terminal restriction fragments (T-RFs) were significantly increased after scFOS administration. Phylogenetic analysis of the 16S rRNA partial gene sequences of murine fecal bacteria suggested that four of six T-RFs that increased after scFOS administration were derived from the 16S rRNA genes of the class Bacteroidetes. Preliminary quantification of Bacteroidetes by real-time PCR suggests that the 16S rRNA genes derived from Bacteroidetes were increased by scFOS administration. Therefore, the T-RFs derived from Bacteroidetes are good markers of change of murine intestinal microbiota after scFOS administration.

These authors contributed equally to this work.

This article has been cited by other articles:

• Collins, R. E., Rocap, G. (2007). REPK: an analytical web server to select restriction endonucleases for terminal restriction fragment length polymorphism analysis. Nucleic Acids Res 35: W58-W62 [Abstract] [Full Text]