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Applied and Environmental Microbiology, September 2006, p. 6377-6380, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00656-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Cloning of a Heavy-Metal-Binding Protein Derived from Activated-Sludge Microorganisms{dagger}

Daisuke Sano, Ken Myojo, and Tatsuo Omura*

Department of Civil Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-06, Sendai 980-8579, Japan

Received 22 March 2006/ Accepted 10 May 2006

A gene of the heavy-metal-binding protein (HMBP) was newly isolated from a genetic DNA library of activated-sludge microorganisms. HMBP was produced by transformed Escherichia coli, and the copper-binding ability of HMBP was confirmed. HMBP derived from activated sludge could be available as heavy metal adsorbents in water and wastewater treatments.

* Corresponding author. Mailing address: Department of Civil Engineering, Graduate School of Engineering, Tohoku University, Aoba 06, Sendai 980-8579, Japan. Phone: 81 22 795 7483. Fax: 81 22 795 7482. E-mail: sano{at}water.civil.tohoku.ac.jp.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2006, p. 6377-6380, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00656-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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