This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stølhaug, A.
Right arrow Articles by Bergh, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stølhaug, A.
Right arrow Articles by Bergh, K.
Agricola
Right arrow Articles by Stølhaug, A.
Right arrow Articles by Bergh, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2006, p. 6394-6398, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02839-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Identification and Differentiation of Legionella pneumophila and Legionella spp. with Real-Time PCR Targeting the 16S rRNA Gene and Species Identification by mip Sequencing

Anne Stølhaug1 and Kåre Bergh1,2*

Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology,1 Department of Medical Microbiology, St. Olavs Hospital, University Hospital of Trondheim, N-7006 Trondheim, Norway2

Received 2 December 2005/ Accepted 16 June 2006

Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established.

* Corresponding author. Mailing address: Department of Medical Microbiology, Laboratory Center, St. Olavs Hospital, N-7006 Trondheim, Norway. Phone: 47 7257 3065. Fax: 47 7257 6416. E-mail: kare.bergh{at}ntnu.no.

Applied and Environmental Microbiology, September 2006, p. 6394-6398, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02839-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Su, H.-P., Tung, S.-K., Tseng, L.-R., Tsai, W.-C., Chung, T.-C., Chang, T. C. (2009). Identification of Legionella Species by Use of an Oligonucleotide Array. J. Clin. Microbiol. 47: 1386-1392 [Abstract] [Full Text]  
  • Chang, B., Sugiyama, K., Taguri, T., Amemura-Maekawa, J., Kura, F., Watanabe, H. (2009). Specific Detection of Viable Legionella Cells by Combined Use of Photoactivated Ethidium Monoazide and PCR/Real-Time PCR. Appl. Environ. Microbiol. 75: 147-153 [Abstract] [Full Text]  
  • Osawa, Y., Ikebukuro, K., Motoki, H., Matsuo, T., Horiuchi, M., Sode, K. (2008). The simple and rapid detection of specific PCR products from bacterial genomes using Zn finger proteins. Nucleic Acids Res 36: e68-e68 [Abstract] [Full Text]  
  • Diederen, B. M. W., Kluytmans, J. A. J. W., Vandenbroucke-Grauls, C. M., Peeters, M. F. (2008). Utility of Real-Time PCR for Diagnosis of Legionnaires' Disease in Routine Clinical Practice. J. Clin. Microbiol. 46: 671-677 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»