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Applied and Environmental Microbiology, September 2006, p. 6424-6428, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02597-05

SHORT REPORT

Molecular-Beacon Multiplex Real-Time PCR Assay for Detection of Vibrio cholerae

Aneta J. Gubala* and David F. Proll

Human Protection and Performance Division, Defence Science and Technology Organisation, Melbourne, Australia

Received 4 November 2005/ Accepted 16 June 2006

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.

* Corresponding author. Mailing address: P.O. Box 4331, Melbourne, Victoria 3001, Australia. Phone: 61 3 9626 8237. Fax: 61 3 9626 8410. E-mail: aneta.gubala{at}dsto.defence.gov.au.

Applied and Environmental Microbiology, September 2006, p. 6424-6428, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02597-05




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  • Fykse, E. M., Skogan, G., Davies, W., Olsen, J. S., Blatny, J. M. (2007). Detection of Vibrio cholerae by Real-Time Nucleic Acid Sequence-Based Amplification. Appl. Environ. Microbiol. 73: 1457-1466 [Abstract] [Full Text]  


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