This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sass, P.
Right arrow Articles by Bierbaum, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sass, P.
Right arrow Articles by Bierbaum, G.
Agricola
Right arrow Articles by Sass, P.
Right arrow Articles by Bierbaum, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2007, p. 347-352, Vol. 73, No. 1
0099-2240/07/$08.00+0     doi:10.1128/AEM.01616-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Lytic Activity of Recombinant Bacteriophage {phi}11 and {phi}12 Endolysins on Whole Cells and Biofilms of Staphylococcus aureus{triangledown}

Peter Sass and Gabriele Bierbaum*

Institute for Medical Microbiology, Immunology and Parasitology (IMMIP), University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany

Received 12 July 2006/ Accepted 27 October 2006

The recombinant {phi}11 endolysin hydrolyzed heat-killed staphylococci as well as staphylococcal biofilms. Cell wall targeting appeared to be a prerequisite for lysis of whole cells, and the combined action of the endopeptidase and amidase domains was necessary for maximum activity. In contrast, the {phi}12 endolysin was inactive and caused aggregation of the cells.

* Corresponding author. Mailing address: Sigmund-Freud-Str. 25, 53105 Bonn, Germany. Phone: (49)228-287-1-9103. Fax: (49)228-287-1-4808. E-mail: bierbaum{at}mibi03.meb.uni-bonn.de.

{triangledown} Published ahead of print on 3 November 2006.

Applied and Environmental Microbiology, January 2007, p. 347-352, Vol. 73, No. 1
0099-2240/07/$08.00+0     doi:10.1128/AEM.01616-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Horgan, M., O'Flynn, G., Garry, J., Cooney, J., Coffey, A., Fitzgerald, G. F., Ross, R. P., McAuliffe, O. (2009). Phage Lysin LysK Can Be Truncated to Its CHAP Domain and Retain Lytic Activity against Live Antibiotic-Resistant Staphylococci. Appl. Environ. Microbiol. 75: 872-874 [Abstract] [Full Text]  
  • Mayer, M. J., Narbad, A., Gasson, M. J. (2008). Molecular Characterization of a Clostridium difficile Bacteriophage and Its Cloned Biologically Active Endolysin. J. Bacteriol. 190: 6734-6740 [Abstract] [Full Text]  
  • Tallent, S. M., Langston, T. B., Moran, R. G., Christie, G. E. (2007). Transducing Particles of Staphylococcus aureus Pathogenicity Island SaPI1 Are Comprised of Helper Phage-Encoded Proteins. J. Bacteriol. 189: 7520-7524 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»