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Applied and Environmental Microbiology, June 2007, p. 3511-3518, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02534-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Flavobacteria Blooms in Four Eutrophic Lakes: Linking Population Dynamics of Freshwater Bacterioplankton to Resource Availability{triangledown} ,{dagger}

Alexander Eiler and Stefan Bertilsson*

Limnology/Department of Ecology and Evolution, Evolutionary Biology Centre, Uppsala University, Norbyv. 20, SE-75236 Uppsala, Sweden

Received 31 October 2006/ Accepted 2 April 2007

Heterotrophic bacteria are major contributors to biogeochemical cycles and influence water quality. Still, the lack of representative isolates and the few quantitative surveys leave the ecological role and significance of single bacterial populations to be revealed. Here we analyzed the diversity and dynamics of freshwater Flavobacteria populations in four eutrophic temperate lakes. From each lake, clone libraries were constructed using primers specific for either the class Flavobacteria or Bacteria. Sequencing of 194 Flavobacteria clones from 8 libraries revealed a diverse freshwater Flavobacteria community and distinct differences among lakes. Abundance and seasonal dynamics of Flavobacteria were assessed by quantitative PCR with class-specific primers. In parallel, the dynamics of individual populations within the Flavobacteria community were assessed with terminal restriction fragment length polymorphism analysis using identical primers. The contribution of Flavobacteria to the total bacterioplankton community ranged from 0.4 to almost 100% (average, 24%). Blooms where Flavobacteria represented more than 30% of the bacterioplankton were observed at different times in the four lakes. In general, high proportions of Flavobacteria appeared during episodes of high bacterial production. Phylogenetic analyses combined with Flavobacteria community fingerprints suggested dominance of two Flavobacteria lineages. Both drastic alterations in total Flavobacteria and in community composition of this class significantly correlated with bacterial production, emphasizing that resource availability is an important driver of heterotrophic bacterial succession in eutrophic lakes.

* Corresponding author. Mailing address: Limnology/Department of Ecology and Evolution, Evolutionary Biology Centre, Uppsala University, Norbyv. 20, SE-75236 Uppsala, Sweden. Phone: 46-18-4712712. Fax: 46-18-531134. E-mail: stebe{at}ebc.uu.se

{triangledown} Published ahead of print on 13 April 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2007, p. 3511-3518, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02534-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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