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Applied and Environmental Microbiology, June 2007, p. 3738-3741, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00390-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

New Insights into the Enterococcus faecalis CroRS Two-Component System Obtained Using a Differential-Display Random Arbitrarily Primed PCR Approach{triangledown}

Yoann Le Breton,*{dagger} Cécile Muller,{dagger} Yanick Auffray, and Alain Rincé

Laboratoire Microbiologie de l'Environnement, EA 956, USC INRA 2017, IRBA, Université de Caen, 14032 Caen Cedex, France

Received 19 February 2007/ Accepted 27 March 2007

Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter.

* Corresponding author. Present address: University of Maryland, Department of Biomedical Sciences, Dental School, 650 West Baltimore Street, Baltimore, MD 21201. Phone: (410) 706-1815. Fax: (410) 706-0865. E-mail: ylebreton{at}umaryland.edu

{triangledown} Published ahead of print on 13 April 2007.

{dagger} Y.L.B. and C.M. contributed equally to this work.

Applied and Environmental Microbiology, June 2007, p. 3738-3741, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00390-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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