This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakai, S.
Right arrow Articles by Kamagata, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakai, S.
Right arrow Articles by Kamagata, Y.
Agricola
Right arrow Articles by Sakai, S.
Right arrow Articles by Kamagata, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2007, p. 4326-4331, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.03008-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Isolation of Key Methanogens for Global Methane Emission from Rice Paddy Fields: a Novel Isolate Affiliated with the Clone Cluster Rice Cluster I{triangledown}

Sanae Sakai,1 Hiroyuki Imachi,1,2* Yuji Sekiguchi,1,3 Akiyoshi Ohashi,1 Hideki Harada,4 and Yoichi Kamagata1,3,5

Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan,1 Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan,2 Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba, Ibaraki 305-8566, Japan,3 Department of Civil Engineering, Tohoku University, Sendai 980-8579, Japan,4 Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Hokkaido 062-8517, Japan5

Received 29 December 2006/ Accepted 1 May 2007

Despite the fact that rice paddy fields (RPFs) are contributing 10 to 25% of global methane emissions, the organisms responsible for methane production in RPFs have remained uncultivated and thus uncharacterized. Here we report the isolation of a methanogen (strain SANAE) belonging to an abundant and ubiquitous group of methanogens called rice cluster I (RC-I) previously identified as an ecologically important microbial component via culture-independent analyses. To enrich the RC-I methanogens from rice paddy samples, we attempted to mimic the in situ conditions of RC-I on the basis of the idea that methanogens in such ecosystems should thrive by receiving low concentrations of substrate (H2) continuously provided by heterotrophic H2-producing bacteria. For this purpose, we developed a coculture method using an indirect substrate (propionate) in defined medium and a propionate-oxidizing, H2-producing syntroph, Syntrophobacter fumaroxidans, as the H2 supplier. By doing so, we significantly enriched the RC-I methanogens and eventually obtained a methanogen within the RC-I group in pure culture. This is the first report on the isolation of a methanogen within RC-I.

* Corresponding author. Mailing address: Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan. Phone: 81-46-867-9709. Fax: 81-46-867-9715. E-mail: imachi{at}jamstec.go.jp

{triangledown} Published ahead of print on 4 May 2007.

Applied and Environmental Microbiology, July 2007, p. 4326-4331, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.03008-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sakai, S., Imachi, H., Sekiguchi, Y., Tseng, I-C., Ohashi, A., Harada, H., Kamagata, Y. (2009). Cultivation of Methanogens under Low-Hydrogen Conditions by Using the Coculture Method. Appl. Environ. Microbiol. 75: 4892-4896 [Abstract] [Full Text]  
  • Cadillo-Quiroz, H., Yavitt, J. B., Zinder, S. H. (2009). Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland. Int. J. Syst. Evol. Microbiol. 59: 928-935 [Abstract] [Full Text]  
  • Robertson, C. E., Spear, J. R., Harris, J. K., Pace, N. R. (2009). Diversity and Stratification of Archaea in a Hypersaline Microbial Mat. Appl. Environ. Microbiol. 75: 1801-1810 [Abstract] [Full Text]  
  • Peng, J., Lu, Z., Rui, J., Lu, Y. (2008). Dynamics of the Methanogenic Archaeal Community during Plant Residue Decomposition in an Anoxic Rice Field Soil. Appl. Environ. Microbiol. 74: 2894-2901 [Abstract] [Full Text]  
  • Sakai, S., Imachi, H., Hanada, S., Ohashi, A., Harada, H., Kamagata, Y. (2008). Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov.. Int. J. Syst. Evol. Microbiol. 58: 929-936 [Abstract] [Full Text]  
  • Cadillo-Quiroz, H., Yashiro, E., Yavitt, J. B., Zinder, S. H. (2008). Characterization of the Archaeal Community in a Minerotrophic Fen and Terminal Restriction Fragment Length Polymorphism-Directed Isolation of a Novel Hydrogenotrophic Methanogen. Appl. Environ. Microbiol. 74: 2059-2068 [Abstract] [Full Text]  
  • Imachi, H., Sakai, S., Sekiguchi, Y., Hanada, S., Kamagata, Y., Ohashi, A., Harada, H. (2008). Methanolinea tarda gen. nov., sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge. Int. J. Syst. Evol. Microbiol. 58: 294-301 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»