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Applied and Environmental Microbiology, August 2007, p. 4791-4796, Vol. 73, No. 15
0099-2240/07/$08.00+0     doi:10.1128/AEM.00120-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evidence of Temporal Postdischarge Decontamination of Bacteria by Gliding Electric Discharges: Application to Hafnia alvei{triangledown}

Georges Kamgang-Youbi,1,2,3 Jean-Marie Herry,1 Marie-Noëlle Bellon-Fontaine,1,4 Jean-Louis Brisset,2 Avaly Doubla,3 and Murielle Naïtali4*

INRA, AgroParisTech, UMR 763 Bioadhésion et Hygiène des Matériaux, 25 Avenue de la République, 91 300 Massy, France,1 Université de Rouen, Laboratoire d'Electrochimie et de Chimie Analytique, UFR de Sciences et Technologies, 76 821 Mont Saint-Aignan, France,2 Université de Yaoundé I, Laboratoire de Chimie Minérale, Département de Chimie Inorganique, B.P. 812, Yaoundé, Cameroon,3 AgroParisTech, INRA, UMR763 Bioadhésion et Hygiène des Matériaux, 1 Avenue des Olympiades, 91 300 Massy, France4

Received 17 January 2007/ Accepted 15 May 2007

This study aimed to characterize the bacterium-destroying properties of a gliding arc plasma device during electric discharges and also under temporal postdischarge conditions (i.e., when the discharge was switched off). This phenomenon was reported for the first time in the literature in the case of the plasma destruction of microorganisms. When cells of a model bacterium, Hafnia alvei, were exposed to electric discharges, followed or not followed by temporal postdischarges, the survival curves exhibited a shoulder and then log-linear decay. These destruction kinetics were modeled using GinaFiT, a freeware tool to assess microbial survival curves, and adjustment parameters were determined. The efficiency of postdischarge treatments was clearly affected by the discharge time (t*); both the shoulder length and the inactivation rate kmax were linearly modified as a function of t*. Nevertheless, all conditions tested (t* ranging from 2 to 5 min) made it possible to achieve an abatement of at least 7 decimal logarithm units. Postdischarge treatment was also efficient against bacteria not subjected to direct discharge, and the disinfecting properties of "plasma-activated water" were dependent on the treatment time for the solution. Water treated with plasma for 2 min achieved a 3.7-decimal-logarithm-unit reduction in 20 min after application to cells, and abatement greater than 7 decimal logarithm units resulted from the same contact time with water activated with plasma for 10 min. These disinfecting properties were maintained during storage of activated water for 30 min. After that, they declined as the storage time increased.

* Corresponding author. Mailing address: UMR763 BHM, AgroparisTech, INRA, 25 Avenue de la République, F-91300 Massy, France. Phone: 33 1 69 53 64 73. Fax: 33 1 69 93 51 44. E-mail: murielle.naitali{at}agroparistech.fr

{triangledown} Published ahead of print on 8 June 2007.

Applied and Environmental Microbiology, August 2007, p. 4791-4796, Vol. 73, No. 15
0099-2240/07/$08.00+0     doi:10.1128/AEM.00120-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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