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Applied and Environmental Microbiology, August 2007, p. 5363-5369, Vol. 73, No. 16
0099-2240/07/$08.00+0     doi:10.1128/AEM.01029-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Saccharomyces cerevisiae Signature Genes for Predicting Nitrogen Deficiency during Alcoholic Fermentation{triangledown} ,{dagger}

A. Mendes-Ferreira,1,2 M. del Olmo,2 J. García-Martínez,3 E. Jiménez-Martí,2 C. Leão,4 A. Mendes-Faia,1* and J. E. Pérez-Ortín2,3

Centro de Genética e Biotecnologia-IBB, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal,1 Departament de Bioquímica i Biologia Molecular, Universitat de València, València, Spain,2 Sección de Chips de DNA, Universitat de València, València, Spain,3 Instituto de Investigação em Ciências da Vida e Saúde (ICVS), Escola de Ciências da Saúde, Universidade do Minho, Braga, Portugal4

Received 9 May 2007/ Accepted 20 June 2007

Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.

* Corresponding author. Mailing address: Centro de Genética e Biotecnologia-IBB, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal. Phone: 351 259350554. Fax: 351 259350480. E-mail: afaia{at}utad.pt

{triangledown} Published ahead of print on 29 June 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2007, p. 5363-5369, Vol. 73, No. 16
0099-2240/07/$08.00+0     doi:10.1128/AEM.01029-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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