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Applied and Environmental Microbiology, August 2007, p. 5382-5384, Vol. 73, No. 16
0099-2240/07/$08.00+0 doi:10.1128/AEM.00066-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Legionella pneumophila in Cooling Towers: Fluctuations in Counts, Determination of Genetic Variability by Pulsed-Field Gel Electrophoresis (PFGE), and Persistence of PFGE Patterns
Sonia Ragull,1
Marian Garcia-Nuñez,1
Maria Luisa Pedro-Botet,1
Nieves Sopena,1
Maria Esteve,2
Rafael Montenegro,3 and
Miquel Sabrià1*
Infectious Diseases Unit,1
Preventive Medicine Service, Hospital Universitari Germans Trias i Pujol, Autonomous University of Barcelona, Badalona, Spain,2
Water Service and Technology Division, Proquimia, Vic, Barcelona, Spain3
Received 11 January 2007/
Accepted 21 June 2007
The concentrations of Legionella pneumophila in cooling towers may vary considerably over short periods of time, producing significant fluctuations throughout the year. Despite genetic variability, in small geographical areas the same indistinguishable pulsed-field gel electrophoresis patterns may be shared among different cooling towers and persist over time.
* Corresponding author. Mailing address: Infectious Diseases Unit, Germans Trias i Pujol University Hospital, Cta Canyet s/n, 08916 Badalona (Barcelona), Spain. Phone: 34 93 497 88 26. Fax: 34 93 497 88 43. E-mail:
msabria.germanstrias{at}gencat.net
Published ahead of print on 29 June 2007.
Applied and Environmental Microbiology, August 2007, p. 5382-5384, Vol. 73, No. 16
0099-2240/07/$08.00+0 doi:10.1128/AEM.00066-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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