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Applied and Environmental Microbiology, September 2007, p. 5471-5476, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.02707-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of D-Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols{triangledown}

Mervi H. Toivari,1* Laura Ruohonen,1 Andrei N. Miasnikov,2 Peter Richard,1 and Merja Penttilä1

VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Espoo, Finland,1 Danisco Global Innovation, Sokeritehtaantie 20, FI-02460 Kantvik, Finland2

Received 20 November 2006/ Accepted 4 July 2007

Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, D-ribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

* Corresponding author. Mailing address: VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Espoo, Finland. Phone: 358 20 722 7309. Fax: 358 20 722 7071. E-mail: Mervi.Toivari{at}vtt.fi

{triangledown} Published ahead of print on 13 July 2007.

Applied and Environmental Microbiology, September 2007, p. 5471-5476, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.02707-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nevoigt, E. (2008). Progress in Metabolic Engineering of Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 72: 379-412 [Abstract] [Full Text]  


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