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Applied and Environmental Microbiology, September 2007, p. 5692-5697, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00419-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Pathotyping Escherichia coli by Using Miniaturized DNA Microarrays{triangledown} ,{dagger}

Muna F. Anjum,1* Muriel Mafura,1 Peter Slickers,2 Karin Ballmer,3 Peter Kuhnert,3 Martin J. Woodward,1 and Ralf Ehricht2

Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom,1 CLONDIAG Chip Technologies GmbH, Jena, Germany,2 Institute of Veterinary Bacteriology, Vetsuisse Faculty of the University of Bern, Bern, Switzerland3

Received 22 February 2007/ Accepted 4 July 2007

The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.

* Corresponding author. Mailing address: Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 (0)1932 359476. Fax: 44 (0)1932 357268. E-mail: m.anjum{at}vla.defra.gsi.gov.uk

{triangledown} Published ahead of print on 13 July 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2007, p. 5692-5697, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00419-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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