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Applied and Environmental Microbiology, September 2007, p. 5857-5864, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00072-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Siderophore Production by Pseudomonas stutzeri under Aerobic and Anaerobic Conditions{triangledown}

Sofia A. Essén,1 Anna Johnsson,2 Dan Bylund,1* Karsten Pedersen,2 and Ulla S. Lundström1

Department of Natural Sciences, Mid Sweden University, SE-851 70 Sundsvall, Sweden,1 Department of Cell and Molecular Biology, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden2

Received 12 January 2007/ Accepted 17 July 2007

The siderophore production of the facultative anaerobe Pseudomonas stutzeri, strain CCUG 36651, grown under both aerobic and anaerobic conditions, was investigated by liquid chromatography and mass spectrometry. The bacterial strain has been isolated at a 626-m depth at the Äspö Hard Rock Laboratory, where experiments concerning the geological disposal of nuclear waste are performed. In bacterial culture extracts, the iron in the siderophore complexes was replaced by gallium to facilitate siderophore identification by mass spectrometry. P. stutzeri was shown to produce ferrioxamine E (nocardamine) as the main siderophore together with ferrioxamine G and two cyclic ferrioxamines having molecular masses 14 and 28 atomic mass units lower than that of ferrioxamine E, suggested to be ferrioxamine D2 and ferrioxamine X1, respectively. In contrast, no siderophores were observed from anaerobically grown P. stutzeri. None of the siderophores produced by aerobically grown P. stutzeri were found in anaerobic natural water samples from the Äspö Hard Rock Laboratory.

* Corresponding author. Mailing address: Department of Natural Sciences, Mid Sweden University, SE-851 70 Sundsvall, Sweden. Phone: 46 60 148909. Fax: 46 60 148820. E-mail: dan.bylund{at}miun.se

{triangledown} Published ahead of print on 3 August 2007.

Applied and Environmental Microbiology, September 2007, p. 5857-5864, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00072-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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