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Applied and Environmental Microbiology, October 2007, p. 6321-6325, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00606-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Use of the tna Operon as a New Molecular Target for Escherichia coli Detection{triangledown}

Camilla Bernasconi,1,{dagger} Giorgio Volponi,2,{dagger} Claudia Picozzi,2* and Roberto Foschino2

European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (VA), Italy,1 DiSTAM, Università degli Studi di Milano, Milano, Italy2

Received 16 March 2007/ Accepted 2 August 2007

A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon.

* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, via Celoria 2, 20133 Milano, Italy. Phone: 39 02 5031 9174. Fax: 39 02 5031 9191. E-mail: Claudia.Picozzi{at}unimi.it

{triangledown} Published ahead of print on 10 August 2007.

{dagger} Both the authors contributed to the work at the same level.

Applied and Environmental Microbiology, October 2007, p. 6321-6325, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00606-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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