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Applied and Environmental Microbiology, January 2007, p. 545-553, Vol. 73, No. 2
0099-2240/07/$08.00+0     doi:10.1128/AEM.01496-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of a Fructose Phosphotransferase System in Bifidobacterium breve UCC2003{triangledown}

Alain Mazé,1,2 Mary O'Connell-Motherway,1 Gerald F. Fitzgerald,1 Josef Deutscher,2 and Douwe van Sinderen1*

Department of Microbiology and Alimentary Pharmabiotic Centre, University of Ireland, Cork, Western Road, Cork, Ireland,1 Laboratoire de Microbiologie et Génétique Moléculaire, INRA-CNRS-INA PG, CBAI, route de Thiverval, 78850 Thiverval-Grignon, France2

Received 29 June 2006/ Accepted 31 October 2006

In silico analysis of the Bifidobacterium breve UCC2003 genome allowed identification of four genetic loci, each of which specifies a putative enzyme II (EII) protein of a phosphoenolpyruvate:sugar phosphotransferase system. The EII encoded by fruA, a clear homologue of the unique EIIBCA enzyme encoded by the Bifidobacterium longum NCC2705 genome, was studied in more detail. The fruA gene is part of an operon which contains fruT, which is predicted to encode a homologue of the Bacillus subtilis antiterminator LicT. Transcriptional analysis showed that the fru operon is induced by fructose. The genetic structure, complementation studies, and the observed transcription pattern of the fru operon suggest that the EII encoded in B. breve is involved in fructose transport and that its expression is controlled by an antiterminator mechanism. Biochemical studies unequivocally demonstrated that FruA phosphorylates fructose at the C-6 position.

* Corresponding author. Mailing address: Department of Microbiology, University of Ireland, Cork, Western Road, Cork, Ireland. Phone: (353) 21 490 1365. Fax: (353) 21 490 3101. E-mail: d.vansinderen{at}ucc.ie.

{triangledown} Published ahead of print on 10 November 2006.

Applied and Environmental Microbiology, January 2007, p. 545-553, Vol. 73, No. 2
0099-2240/07/$08.00+0     doi:10.1128/AEM.01496-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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