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Applied and Environmental Microbiology, November 2007, p. 6905-6909, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00971-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Indirect Oxidation of Co(II) in the Presence of the Marine Mn(II)-Oxidizing Bacterium Bacillus sp. Strain SG-1{triangledown}

Karen J. Murray,1 Samuel M. Webb,2 John R. Bargar,2 and Bradley M. Tebo1,3*

Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0202,1 Stanford Synchrotron Radiation Laboratory, Stanford Linear Accelerator Center, Menlo Park, California 94025,2 Department of Environmental and Biomolecular Systems, OGI School of Science & Engineering, Oregon Health & Science University, 2000 NW Walker Rd., Beaverton, Oregon 970063

Received 30 April 2007/ Accepted 30 August 2007

Cobalt(II) oxidation in aquatic environments has been shown to be linked to Mn(II) oxidation, a process primarily mediated by bacteria. This work examines the oxidation of Co(II) by the spore-forming marine Mn(II)-oxidizing bacterium Bacillus sp. strain SG-1, which enzymatically catalyzes the formation of reactive nanoparticulate Mn(IV) oxides. Preparations of these spores were incubated with radiotracers and various amounts of Co(II) and Mn(II), and the rates of Mn(II) and Co(II) oxidation were measured. Inhibition of Mn(II) oxidation by Co(II) and inhibition of Co(II) oxidation by Mn(II) were both found to be competitive. However, from both radiotracer experiments and X-ray spectroscopic measurements, no Co(II) oxidation occurred in the complete absence of Mn(II), suggesting that the Co(II) oxidation observed in these cultures is indirect and that a previous report of enzymatic Co(II) oxidation may have been due to very low levels of contaminating Mn. Our results indicate that the mechanism by which SG-1 oxidizes Co(II) is through the production of the reactive nanoparticulate Mn oxide.

* Corresponding author. Mailing address: Department of Environmental and Biomolecular Systems, OGI School of Science & Engineering, Oregon Health & Science University, 20000 NW Walker Rd., Beaverton, OR 97006. Phone: (503) 748-1992. Fax: (503) 748-1464. E-mail: tebo{at}ebs.ogi.edu

{triangledown} Published ahead of print on 7 September 2007.

Applied and Environmental Microbiology, November 2007, p. 6905-6909, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00971-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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