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Applied and Environmental Microbiology, November 2007, p. 7092-7095, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.01394-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Construction of p16Slux, a Novel Vector for Improved Bioluminescent Labeling of Gram-Negative Bacteria{triangledown}

Christian U. Riedel,1,2,{dagger} Pat G. Casey,1,2 Heidi Mulcahy,2,3,{ddagger} Fergal O'Gara,2,3 Cormac G. M. Gahan,1,2,4* and Colin Hill1,2

Alimentary Pharmabiotic Centre,1 Department of Microbiology,2 BIOMERIT Research Centre,3 School of Pharmacy, University College Cork, Cork, Ireland4

Received 22 June 2007/ Accepted 7 August 2007

A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.

* Corresponding author. Mailing address: Department of Microbiology, University College Cork, Cork, Ireland. Phone: 353 (0)21 4901363. Fax: 353 (0)21 4903101. E-mail: c.gahan{at}ucc.ie

{triangledown} Published ahead of print on 31 August 2007.

{dagger} Present address: Institute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany.

{ddagger} Present address: Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1.

Applied and Environmental Microbiology, November 2007, p. 7092-7095, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.01394-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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