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Applied and Environmental Microbiology, November 2007, p. 7471-7473, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.00978-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Environmental Monitoring of Mycobacterium bovis in Badger Feces and Badger Sett Soil by Real-Time PCR, as Confirmed by Immunofluorescence, Immunocapture, and Cultivation{triangledown}

F. P. Sweeney,1* O. Courtenay,2 V. Hibberd,1 R. G. Hewinson,3 L. A. Reilly,2 W. H. Gaze,1 and E. M. H. Wellington1

Microbiology Group,1 Ecology and Epidemiology Group, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom,2 Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom3

Received 1 May 2007/ Accepted 16 September 2007

Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, United Kingdom. Phone: 44 (0)2476 522431. Fax: 44 (0)2476 523701. E-mail: f.p.sweeney{at}warwick.ac.uk

{triangledown} Published ahead of print on 28 September 2007.

Applied and Environmental Microbiology, November 2007, p. 7471-7473, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.00978-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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