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Applied and Environmental Microbiology, November 2007, p. 7488-7493, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01112-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Design and Performance of a 16S rRNA-Targeted Oligonucleotide Probe for Detection of Members of the Genus Bdellovibrio by Fluorescence In Situ Hybridization{triangledown}

Khaled K. Mahmoud, Damian McNeely, Chelsea Elwood, and Susan F. Koval*

Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1

Received 17 May 2007/ Accepted 17 September 2007

A 16S rRNA-targeted, Cy3-labeled oligonucleotide probe was designed to detect members of the genus Bdellovibrio by fluorescence in situ hybridization. Specific hybridization conditions were established; however, the detection of bdellovibrios in environmental samples required enrichment, confirming that Bdellovibrio spp. are not present in large numbers in the environment.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1. Phone: (519) 661-3439. Fax: (519) 661-3499. E-mail: skoval{at}uwo.ca

{triangledown} Published ahead of print on 28 September 2007.

Applied and Environmental Microbiology, November 2007, p. 7488-7493, Vol. 73, No. 22
0099-2240/07/$08.00+0     doi:10.1128/AEM.01112-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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