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Applied and Environmental Microbiology, December 2007, p. 7605-7614, Vol. 73, No. 23
0099-2240/07/$08.00+0     doi:10.1128/AEM.01225-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Different Genotypes of Anatoxin-Producing Cyanobacteria Coexist in the Tarn River, France{triangledown} ,{dagger}

Sabrina Cadel-Six,1,{ddagger} Caroline Peyraud-Thomas,1,{ddagger} Luc Brient,2 Nicole Tandeau de Marsac,1 Rosmarie Rippka,1 and Annick Méjean1*

Unité des Cyanobactéries (CNRS URA 2172), Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France,1 UMR Eco-Bio, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, France2

Received 1 June 2007/ Accepted 4 October 2007

Repeated dog deaths occurred in 2002, 2003, and 2005 after the animals drank water from the shoreline of the Tarn River in southern France. Signs of intoxication indicated acute poisoning due to a neurotoxin. Floating scum and biofilms covering pebbles were collected in the summers of 2005 and 2006 from six different sites along 30 km from the border of this river. The cyanobacterial neurotoxic alkaloid anatoxin-a and/or its methyl homolog, homoanatoxin-a, was detected in the extracts of most samples examined by gas chromatography-mass spectrometry. Fifteen filamentous cyanobacteria of the order Oscillatoriales were isolated and displayed four distinct phenotypes based on morphological characteristics and pigmentation. Three of the phenotypes can be assigned to the genus Oscillatoria or Phormidium, depending on the taxonomic treatises (bacteriological/botanical) employed for identification. The fourth phenotype is typical of the genus Geitlerinema Anagnostidis 1989. Eight strains rendered axenic were analyzed for production of anatoxin-a and homoanatoxin-a, and all strains of Oscillatoria/Phormidium proved to be neurotoxic. The genetic relatedness of the new isolates was evaluated by comparison of the intergenic transcribed spacer sequences with those of six oscillatorian strains from the Pasteur Culture Collection of Cyanobacteria. These analyses showed that the neurotoxic representatives are composed of five different genotypes, three of which correspond to phenotypes isolated in this study. Our findings prove that neurotoxic oscillatorian cyanobacteria exist in the Tarn River and thus were most likely implicated in the reported dog poisonings. Furthermore, they reemphasize the importance of monitoring benthic cyanobacteria in aquatic environments to fully assess the health risks associated with these organisms.

* Corresponding author. Mailing address: Unité des Cyanobactéries (CNRS URA 2172), Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 40 61 32 81. Fax: 33 1 40 61 30 42. E-mail: amejean{at}pasteur.fr

{triangledown} Published ahead of print on 12 October 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Equal first authors.

Applied and Environmental Microbiology, December 2007, p. 7605-7614, Vol. 73, No. 23
0099-2240/07/$08.00+0     doi:10.1128/AEM.01225-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cadel-Six, S., Iteman, I., Peyraud-Thomas, C., Mann, S., Ploux, O., Mejean, A. (2009). Identification of a Polyketide Synthase Coding Sequence Specific for Anatoxin-a-Producing Oscillatoria Cyanobacteria. Appl. Environ. Microbiol. 75: 4909-4912 [Abstract] [Full Text]  
  • Bruno, L., Billi, D., Bellezza, S., Albertano, P. (2009). Cytomorphological and Genetic Characterization of Troglobitic Leptolyngbya Strains Isolated from Roman Hypogea. Appl. Environ. Microbiol. 75: 608-617 [Abstract] [Full Text]  


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