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Applied and Environmental Microbiology, December 2007, p. 7814-7818, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01140-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Engineered Synthetic Pathway for Isopropanol Production in Escherichia coli{triangledown} ,{dagger}

T. Hanai,{ddagger} S. Atsumi, and J. C. Liao*

Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, California 90095

Received 22 May 2007/ Accepted 6 October 2007

A synthetic pathway was engineered in Escherichia coli to produce isopropanol by expressing various combinations of genes from Clostridium acetobutylicum ATCC 824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593, and Thermoanaerobacter brockii HTD4. The strain with the combination of C. acetobutylicum thl (acetyl-coenzyme A [CoA] acetyltransferase), E. coli atoAD (acetoacetyl-CoA transferase), C. acetobutylicum adc (acetoacetate decarboxylase), and C. beijerinckii adh (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in E. coli, and the titer exceeded that from the native producers.

* Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, CA 90095. Phone: (310) 825-1656. Fax: (310) 206-4107. E-mail: liaoj{at}ucla.edu

{triangledown} Published ahead of print on 12 October 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Permanent address: Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Applied and Environmental Microbiology, December 2007, p. 7814-7818, Vol. 73, No. 24
0099-2240/07/$08.00+0     doi:10.1128/AEM.01140-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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