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Applied and Environmental Microbiology, February 2007, p. 1256-1265, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01766-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Effectiveness of Disinfectants in Killing Enterobacter sakazakii in Suspension, Dried on the Surface of Stainless Steel, and in a Biofilm{triangledown}

Hoikyung Kim,1 Jee-Hoon Ryu,2* and Larry R. Beuchat1*

Center for Food Safety and Department of Food Science and Technology, University of Georgia, 1109 Experiment Street, Griffin, Georgia,1 Graduate School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul 136-791, Republic of Korea2

Received 26 July 2006/ Accepted 5 December 2006

The effectiveness of 13 disinfectants used in hospitals, day-care centers, and food service kitchens in killing Enterobacter sakazakii in suspension, dried on the surface of stainless steel, and in biofilm was determined. E. sakazakii exhibited various levels of resistance to the disinfectants, depending on the composition of the disinfectants, amount and type of organic matrix surrounding cells, and exposure time. Populations of planktonic cells suspended in water (7.22 to 7.40 log CFU/ml) decreased to undetectable levels (<0.30 log CFU/ml) within 1 to 5 min upon treatment with disinfectants, while numbers of cells in reconstituted infant formula were reduced by only 0.02 to 3.69 log CFU/ml after the treatment for 10 min. The presence of infant formula also enhanced the resistance to the disinfectants of cells dried on the surface of stainless steel. The resistance of cells to disinfectants in 6-day-old and 12-day-old biofilms on the surface of stainless steel was not significantly different. The overall order of efficacy of disinfectants in killing E. sakazakii was planktonic cells > cells inoculated and dried on stainless steel > cells in biofilms on stainless steel. Findings show that disinfectants routinely used in hospital, day-care, and food service kitchen settings are ineffective in killing some cells of E. sakazakii embedded in organic matrices.

* Corresponding author. Mailing address for Larry R. Beuchat: Center for Food Safety, University of Georgia, 1109 Experiment Street, Griffin, GA 30223-1797. Phone: (770) 412-4740. Fax: (770) 229-3216. E-mail: lbeuchat{at}uga.edu. Mailing address for Jee-Hoon Ryu: Graduate School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul 136-791, Republic of Korea. Phone: 82 2 3290 3409. Fax: 82 2 3290 3918. E-mail address: escheri{at}korea.ac.kr.

{triangledown} Published ahead of print on 15 December 2006.

Applied and Environmental Microbiology, February 2007, p. 1256-1265, Vol. 73, No. 4
0099-2240/07/$08.00+0     doi:10.1128/AEM.01766-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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