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Applied and Environmental Microbiology, March 2007, p. 2001-2004, Vol. 73, No. 6
0099-2240/07/$08.00+0     doi:10.1128/AEM.02515-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Biochemical Characterization of an L-Xylulose Reductase from Neurospora crassa{triangledown} ,{dagger}

Nikhil Nair1 and Huimin Zhao1,2,3,4*

Departments of Chemical and Biomolecular Engineering,1 Chemistry,2 Center for Biophysics and Computational Biology,3 Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, Illinois 618014

Received 28 October 2006/ Accepted 8 January 2007

An L-xylulose reductase identified from the genome sequence of the filamentous fungus Neurospora crassa was heterologously expressed in Escherichia coli as a His6 tag fusion protein, purified, and characterized. The enzyme may be used in the production of xylitol from the major pentose components of hemicellulosic waste, D-xylose and L-arabinose.

* Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 215 RAL, Box C3, 600 S. Mathews Ave., Urbana, IL 61801. Phone: (217) 333-2631. Fax: (217) 333-5052. E-mail: zhao5{at}uiuc.edu.

{triangledown} Published ahead of print on 19 January 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, March 2007, p. 2001-2004, Vol. 73, No. 6
0099-2240/07/$08.00+0     doi:10.1128/AEM.02515-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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